Experimental laboratory days started between 8.00 and 9.30am with participants having fasted for 10-hour overnight (Figure 1). The day commenced with a standard breakfast, followed by either 3 hours of rest (control condition- CON) or two hours of rest separated by one hour of cycling at 50% of maximal oxygen consumption (exercise condition- EX). Following this 3 hour period, participants consumed an ad libitum lunch and were then provided with a combined heart rate and accelerometer monitor (Actiheart, Cambridge Neurotechnology, Cambridge, UK) and a food diary that were used to estimate energy intake and expenditure over the following 3 days.