Data from: Investigation of factors influencing the hydrolytic degradation of single PLGA microparticles

Summary

Abstract Poly lactide-co-glycolide (PLGA) is an important polymer matrix used to provide sustained release across a range of active pharmaceutical ingredients (APIs) and works by hydrolytic degradation within the body, thereby releasing entrapped drug. Processing and sterilisation can impact on the morphology and chemistry of PLGA therefore influencing the hydrolysis rate and in turn the release rate of any entrapped API. This paper has looked at the effect of supercritical carbon dioxide (scCO2) processing, gamma irradiation, comonomer ratio and temperature on the hydrolysis of individual PLGA microparticles, using a combination of Attenuated Total Reflectance-Fourier Transform Infrared (ATR-FTIR) imaging, Scanning Electron Microscopy (SEM), Differential Scanning Calorimetery (DSC) and Gel Permeation chromatography (GPC) to facilitate a better understanding of the physiochemical factors affecting the hydrolysis rate. This work has shown that scCO2 processing influences hydrolysis rates by increasing the porosity of the PLGA microparticles, increasing the lactide comonomer ratio decreases hydrolysis rates by reducing the hydrophilicity of the PLGA microparticles and increasing the gamma irradiation dose systematically increases the rate of hydrolysis due to reducing the overall molecular weight of the polymer matrix via a chain scission mechanism. Moreover this work shows that ATR-FTIR imaging facilitates the determination of a range of physicochemical parameters during the hydrolysis of a single PLGA microparticle including water ingress, water/polymer interface dimensions, degradation product distribution and hydrolysis rates for both lactide and glycolide copolymer units from the same experiment.

Keywords:	Polymer degradation, PLGA, Controlled release, ATR-FTIR spectroscopic imaging, Gamma-irradiation, Degradation rate
Creators:	Keles, Hakan http://orcid.org/0000-0002-5204-3841 Naylor, Andrew Clegg, Francis Sammon, Chris http://orcid.org/0000-0003-1714-1726
Academic units:	Faculty of Science, Technology and Arts (STA) > Research Centres > Materials and Engineering Research Institute (MERI) > Polymers, Nanocomposites and Modelling Research Centre
Funders:	Funder Name Grant Number Funder ID Engineering and Physical Sciences Research Council EP/15016654/1 http://dx.doi.org/10.13039/501100000266
Copyright Holders:	Sheffield Hallam University
Publisher of the data:	SHU Research Data Archive (SHURDA)
Publication date:	13 October 2015
Data last accessed:	15 February 2017
DOI:	http://doi.org/10.17032/shu-150002
SHURDA URI:	http://shurda.shu.ac.uk/id/eprint/4
Additional details Additional details Types of data: Dataset Collection period: From To October 2011 September 2014 Data collection method: Data collected using a Varian FT690 FTIR instrument coupled to a 64x64 FPA detector with a Graseby single reflection ATR sampling accessory. Data processing and preparation activities: Raw data converted to .spc format. Resource language: English Metadata language: English Depositing User: Chris Sammon Date Deposited: 13 Oct 2015 13:59 Last Modified: 13 Oct 2015 15:24